Saccharomyces cerevisiae is the principal yeast responsible for the formation of wine from grapes, and increasingly an important model for genetics and species adaptation to ecological niches. The distribution of S. cerevisiae in natural and agricultural environments is thought to be random, with little adaptation to specific niches, but the influence and selection of the common insect group, Drosopholids, as vectors has not been considered. The vineyard provides an important interface between the ecotypes found in arboreal and human habitats, and with this in mind, this study purified over 100 isolates of S. cerevisiae from within vineyard and in the surrounding natural vegetation in France. Soil, insects, flowers, grapes and bark were sampled, S. cerevisiae was enriched and pure colonies genotyped and common phenotypes assessed. In some cases, isolates were closely affiliated with ‘wild’ isolates, but other isolates were found in two or more microhabitats. The isolates were differently attractive to Drosophila melanogaster and Drosophila suzukii, as found by an oviposition assay, which correlated with production of aromatic compounds. Our results demonstrate the importance of insect vectors to transport, maintain and differentiate ecological niches for the well-described yeast, S. cerevisiae.