In the East Malaysian State of Sabah, melioidosis fatalities have been recorded in both human and livestock for at least more than five decades. Prior to the existence of any formal inter-agency collaboration between the Sabah State Health Department (SSHD) and the Sabah State Department of Veterinary Services & Animal Industry (SSDVSAI), death of farm animal due to melioidosis rarely raised much concerns of the increased risk of infection with melioidosis amongst farm personnel. We describe here how the establishment of inter-agency collaboration between SSHD and SSDVSAI may have helped avert a potential melioidosis outbreak among farm personnel. In May 2016, the Biotechnology Centre of SSDVSAI reported the death of a Saanen goat. The melioidosis causative organism, Burkholderia pseudomallei was isolated from the organs of the dead goat. As part of the inter-agency collaboration, SSDVSAI alerted SSHD of the diagnostic outcome. Subsequently in September 2016, SSHD conducted health check on all the farm personnel at the center as well as two other separate goat farms that have also reported animal cases suggestive of melioidosis. All farm personnel were examined for symptoms suggestive of melioidosis and 35 blood samples were collected to determine exposure to the disease. 49 Soil and 17 water samples were collected from around the premises of the centre and the farms to determine the presence of B. pseudomallei in the environment. 4 blood samples were seropositive for melioidosis IgM as determined using ELISA. Only one soil sample was positive for B. pseudomallei, isolated using direct culture method. The four personnel with seropositive reactions have since been referred to Infectious Disease specialist for further monitoring and follow-up. All farm personnel were also being briefed about the disease as part of prevention and control measures. These events highlight the importance of the One-Health collaborative measures in controlling zoonotic diseases in Sabah.